Waterra eDNA Filter Shaker

Description

waterra eDNA Filter Shaker

The waterra eDNA Filter shaker is a hand held tool used to process eDNA Filter samples in the field. The shaker agitates the injected buffer within the filter itself exposing the buffer to eDNA that was trapped in the filter media during the sampling phase.

Waterra eDNA filters are advantageous because of the large surface area of the filter media which allows the processing of large amounts of water. This can be helpful for tracking low density eDNA targets.

Sample Collection Techniques:
The suggested process is to connect a suction type pump (waterra has options to consider) to the outlet of the filter and draw water in through the filter. This traps water borne eDNA in the filter media.

Sample Processing in the Field:
After the requisite volume is pumped through the waterra eDNA filter, end caps/plugs are inserted into one end of the filter. A volume of buffer is injected using a waterra syringe and syringe adapter into the other end of the filter. The second end is capped or plugged, trapping the buffer in the filter. The filter is then inserted into the filter holder ring aligning the gussets under the filter end with gaps in the ring.

Holding the Shaker horizontal, the tool is activated with a finger trigger for 15 seconds or so. Then slide the filter out a bit, rotate it 90 degrees to the next set of gussets and activate the trigger for another 15 seconds.

Extracting the Buffer:
Remove the plug or end cap from the opposite side of the filter that received the buffer. Using the waterra syringe and syringe adapter force air into the filter pushing the buffer out the opposite end into a sample container.

Filter Buffer End Caps
Waterra eDNA filters come with 2 end caps/plugs to securely contain eDNA preserving buffers in the filter capsule.

Learn More about Waterra eDNA Filters